STIMATE Membrane Protein Introduction

Introduction of STIMATE

TMEM110, also designated as STIMATE, is an ER-resident multi-transmembrane protein identified through a proteomic study on the ER-PM junctions. The ER-PM junctions are defined as specialized junctional sites, also known as membrane contact sites that connect the endoplasmic reticulum (ER) and the plasma membrane (PM), and are closely implicated in controlling lipid and calcium homeostasis in mammalian cells.

Basic Information of STIMATE
Protein Name	Store-operated calcium entry regulator STIMATE
Gene Name	STIMATE
Aliases	TMEM110
Organism	Homo sapiens (Human)
UniProt ID	Q86TL2
Transmembrane Times	5
Length (aa)	294
Sequence	MQGPAGNASRGLPGGPPSTVASGAGRCESGALMHSFGIFLQGLLGVVAFSTLMLKRFREPKHERRPWRIWFLDTSKQAIGMLFIHFANVYLADLTEEDPCSLYLINFLLDATVGMLLIYVGVRAVSVLVEWQQWESLRFGEYGDPLQCGAWVGQCALYIVIMIFEKSVVFIVLLILQWKKVALLNPIENPDLKLAIVMLIVPFFVNALMFWVVDNFLMRKGKTKAKLEERGANQDSRNGSKVRYRRAASHEESESEILISADDEMEESDVEEDLRRLTPLKPVKKKKHRFGLPV

Function of STIMATE Membrane Protein

TMEM110 is a positive modulator of calcium flux mediated by the STIM-ORAI signaling in vertebrates. STIMATE can physically associate with STIM1 to promote conformational switch of STIM1 from inactive toward an activated state, thereby coupling to and gating the ORAI calcium channels on the plasma membrane. Depletion of TMEM110 with RNAi knockdown or Cas9-mediated gene disruption substantially reduces the puncta formation of STIM1 at ER-PM junctions and remarkably inhibits the calcium/calcineurin/ NFAT signaling axis. Genetic depletion of STIMATE substantially reduces STIM1 puncta formation at ER-PM junctions and suppresses the Ca²⁺-NFAT signaling.

STIMATE Membrane Protein Introduction Fig.1 Tentative model of channels and Na⁺ /H⁺ ion exchanger in cell proliferation. (Lang, 2013)

Application of STIMATE Membrane Protein in Literature

Song J., et al. Integrated pipeline for inferring the evolutionary history of a gene family embedded in the species tree: a case study on the STIMATE gene family. BMC Bioinformatics. 2017, 18(1): 439. PubMed ID: 28974198

This study inferred STIMATE (TMEM110) gene family phylogenetic trees in 69 sequenced chordate genomes by an integrated pipeline.
Quintana A., et al. TMEM110 regulates the maintenance and remodeling of mammalian ER-plasma membrane junctions competent for STIM-ORAI signaling. Proc Natl Acad Sci U S A. 2015, 112(51): E7083-92. PubMed ID: 26644574

Authors reported that an ER-resident membrane protein identified in a previous genome-wide RNAi screen, transmembrane protein 110 (TMEM110), regulated the long-term maintenance of ER-plasma membrane junctions and the short-term physiological remodeling of the junctions during store-dependent calcium signaling.
Jing J., et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca²⁺ influx. Nat Cell Biol. 2015, 17(10): 1339-1347. PubMed ID: 26322679

This study showed that STIMATE, as a regulator of Ca²⁺ influx in vertebrates, promoted STIM1 conformational switch by physically interacting with STIM1.
Hering D.M., et al. Genome-wide association study for semen volume and total number of sperm in Holstein-Friesian bulls. Mol Endocrinol. 2014, 151(3-4): 126-130. PubMed ID: 25465359

This study was to estimate the effect of SNP marker for semen volume (SV) and total number of sperm (TNS) and identified three genes (DCP1A, SFMBT1, TMEM110) located near some significant markers.

STIMATE Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-STIMATE antibody development services.

Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.

Reference

Lang F, et al. (2013). Ion channels in cancer: future perspectives and clinical potential. Philos Trans R Soc Lond B Biol Sci. 2014, 369(1638), 20130108.

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